Review



acumen e x3 microplate cytometer  (SPT Labtech)


Bioz Verified Symbol SPT Labtech is a verified supplier
Bioz Manufacturer Symbol SPT Labtech manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    SPT Labtech acumen e x3 microplate cytometer
    EPHA2 receptor specifically mediates targeted adenoviral vector internalisation into OS cells. ( A ) Representative fluorescence images of SaOS-2 and MG-63 OS cells and Hum63 hp-OBs subjected to transduction with AdYSA at the indicated MOIs, acquired using the <t>Acumen</t> <t>e</t> <t>X3</t> <t>microplate</t> cytometer. Composite images show Hoechst-stained cell nuclei (blue) and GFP-expression (green). ( B ) Transduction of OS cell lines (green bars) and hp-OBs (blue bars) with EPHA2-targeted adenoviral vector AdYSA, as measured by GFP expression 48 h after subjecting the cells to virus at MOI-100. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. The observed difference between OS cells and hp-OBs is three- to six-fold (Student's t -test; * P <0.05). ( C ) Competition by pre-incubation of the cells with synthetic peptides before incubation with AdYSA. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. Green bars represent the control condition in which the cells were incubated with AdYSA alone, grey bars represent the control condition in which cells were pre-incubated with Cys.S peptide, followed by incubation with AdYSA and blue bars represent the competition condition in which cells were pre-incubated with YSA peptide followed by incubation with AdYSA. Receptor blocking with YSA peptide results in significant reduction of transduction efficiency in the OS cell lines (**** P <0.001 for SaOS-2; *** P <0.005 for LM7 and U2OS; ** P <0.01 for MG-63), but not in the already poorly transduced healthy bone cells (NS).
    Acumen E X3 Microplate Cytometer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acumen+e+x3+microplate+cytometer/pmc03798973-132-12-17?v=SPT+Labtech
    Average 86 stars, based on 1 article reviews
    acumen e x3 microplate cytometer - by Bioz Stars, 2026-07
    86/100 stars

    Images

    1) Product Images from "Surface proteomic analysis of osteosarcoma identifies EPHA2 as receptor for targeted drug delivery"

    Article Title: Surface proteomic analysis of osteosarcoma identifies EPHA2 as receptor for targeted drug delivery

    Journal: British Journal of Cancer

    doi: 10.1038/bjc.2013.578

    EPHA2 receptor specifically mediates targeted adenoviral vector internalisation into OS cells. ( A ) Representative fluorescence images of SaOS-2 and MG-63 OS cells and Hum63 hp-OBs subjected to transduction with AdYSA at the indicated MOIs, acquired using the Acumen e X3 microplate cytometer. Composite images show Hoechst-stained cell nuclei (blue) and GFP-expression (green). ( B ) Transduction of OS cell lines (green bars) and hp-OBs (blue bars) with EPHA2-targeted adenoviral vector AdYSA, as measured by GFP expression 48 h after subjecting the cells to virus at MOI-100. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. The observed difference between OS cells and hp-OBs is three- to six-fold (Student's t -test; * P <0.05). ( C ) Competition by pre-incubation of the cells with synthetic peptides before incubation with AdYSA. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. Green bars represent the control condition in which the cells were incubated with AdYSA alone, grey bars represent the control condition in which cells were pre-incubated with Cys.S peptide, followed by incubation with AdYSA and blue bars represent the competition condition in which cells were pre-incubated with YSA peptide followed by incubation with AdYSA. Receptor blocking with YSA peptide results in significant reduction of transduction efficiency in the OS cell lines (**** P <0.001 for SaOS-2; *** P <0.005 for LM7 and U2OS; ** P <0.01 for MG-63), but not in the already poorly transduced healthy bone cells (NS).
    Figure Legend Snippet: EPHA2 receptor specifically mediates targeted adenoviral vector internalisation into OS cells. ( A ) Representative fluorescence images of SaOS-2 and MG-63 OS cells and Hum63 hp-OBs subjected to transduction with AdYSA at the indicated MOIs, acquired using the Acumen e X3 microplate cytometer. Composite images show Hoechst-stained cell nuclei (blue) and GFP-expression (green). ( B ) Transduction of OS cell lines (green bars) and hp-OBs (blue bars) with EPHA2-targeted adenoviral vector AdYSA, as measured by GFP expression 48 h after subjecting the cells to virus at MOI-100. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. The observed difference between OS cells and hp-OBs is three- to six-fold (Student's t -test; * P <0.05). ( C ) Competition by pre-incubation of the cells with synthetic peptides before incubation with AdYSA. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. Green bars represent the control condition in which the cells were incubated with AdYSA alone, grey bars represent the control condition in which cells were pre-incubated with Cys.S peptide, followed by incubation with AdYSA and blue bars represent the competition condition in which cells were pre-incubated with YSA peptide followed by incubation with AdYSA. Receptor blocking with YSA peptide results in significant reduction of transduction efficiency in the OS cell lines (**** P <0.001 for SaOS-2; *** P <0.005 for LM7 and U2OS; ** P <0.01 for MG-63), but not in the already poorly transduced healthy bone cells (NS).

    Techniques Used: Plasmid Preparation, Fluorescence, Transduction, Cytometry, Staining, Expressing, Incubation, Blocking Assay



    Similar Products

    acumen e x3 microplate cytometer  (SPT Labtech)
    86
    SPT Labtech acumen e x3 microplate cytometer
    EPHA2 receptor specifically mediates targeted adenoviral vector internalisation into OS cells. ( A ) Representative fluorescence images of SaOS-2 and MG-63 OS cells and Hum63 hp-OBs subjected to transduction with AdYSA at the indicated MOIs, acquired using the <t>Acumen</t> <t>e</t> <t>X3</t> <t>microplate</t> cytometer. Composite images show Hoechst-stained cell nuclei (blue) and GFP-expression (green). ( B ) Transduction of OS cell lines (green bars) and hp-OBs (blue bars) with EPHA2-targeted adenoviral vector AdYSA, as measured by GFP expression 48 h after subjecting the cells to virus at MOI-100. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. The observed difference between OS cells and hp-OBs is three- to six-fold (Student's t -test; * P <0.05). ( C ) Competition by pre-incubation of the cells with synthetic peptides before incubation with AdYSA. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. Green bars represent the control condition in which the cells were incubated with AdYSA alone, grey bars represent the control condition in which cells were pre-incubated with Cys.S peptide, followed by incubation with AdYSA and blue bars represent the competition condition in which cells were pre-incubated with YSA peptide followed by incubation with AdYSA. Receptor blocking with YSA peptide results in significant reduction of transduction efficiency in the OS cell lines (**** P <0.001 for SaOS-2; *** P <0.005 for LM7 and U2OS; ** P <0.01 for MG-63), but not in the already poorly transduced healthy bone cells (NS).
    Acumen E X3 Microplate Cytometer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/acumen+e+x3+microplate+cytometer/pmc03798973-132-12-17?v=SPT+Labtech
    Average 86 stars, based on 1 article reviews
    acumen e x3 microplate cytometer - by Bioz Stars, 2026-07
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    EPHA2 receptor specifically mediates targeted adenoviral vector internalisation into OS cells. ( A ) Representative fluorescence images of SaOS-2 and MG-63 OS cells and Hum63 hp-OBs subjected to transduction with AdYSA at the indicated MOIs, acquired using the Acumen e X3 microplate cytometer. Composite images show Hoechst-stained cell nuclei (blue) and GFP-expression (green). ( B ) Transduction of OS cell lines (green bars) and hp-OBs (blue bars) with EPHA2-targeted adenoviral vector AdYSA, as measured by GFP expression 48 h after subjecting the cells to virus at MOI-100. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. The observed difference between OS cells and hp-OBs is three- to six-fold (Student's t -test; * P <0.05). ( C ) Competition by pre-incubation of the cells with synthetic peptides before incubation with AdYSA. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. Green bars represent the control condition in which the cells were incubated with AdYSA alone, grey bars represent the control condition in which cells were pre-incubated with Cys.S peptide, followed by incubation with AdYSA and blue bars represent the competition condition in which cells were pre-incubated with YSA peptide followed by incubation with AdYSA. Receptor blocking with YSA peptide results in significant reduction of transduction efficiency in the OS cell lines (**** P <0.001 for SaOS-2; *** P <0.005 for LM7 and U2OS; ** P <0.01 for MG-63), but not in the already poorly transduced healthy bone cells (NS).

    Journal: British Journal of Cancer

    Article Title: Surface proteomic analysis of osteosarcoma identifies EPHA2 as receptor for targeted drug delivery

    doi: 10.1038/bjc.2013.578

    Figure Lengend Snippet: EPHA2 receptor specifically mediates targeted adenoviral vector internalisation into OS cells. ( A ) Representative fluorescence images of SaOS-2 and MG-63 OS cells and Hum63 hp-OBs subjected to transduction with AdYSA at the indicated MOIs, acquired using the Acumen e X3 microplate cytometer. Composite images show Hoechst-stained cell nuclei (blue) and GFP-expression (green). ( B ) Transduction of OS cell lines (green bars) and hp-OBs (blue bars) with EPHA2-targeted adenoviral vector AdYSA, as measured by GFP expression 48 h after subjecting the cells to virus at MOI-100. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. The observed difference between OS cells and hp-OBs is three- to six-fold (Student's t -test; * P <0.05). ( C ) Competition by pre-incubation of the cells with synthetic peptides before incubation with AdYSA. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. Green bars represent the control condition in which the cells were incubated with AdYSA alone, grey bars represent the control condition in which cells were pre-incubated with Cys.S peptide, followed by incubation with AdYSA and blue bars represent the competition condition in which cells were pre-incubated with YSA peptide followed by incubation with AdYSA. Receptor blocking with YSA peptide results in significant reduction of transduction efficiency in the OS cell lines (**** P <0.001 for SaOS-2; *** P <0.005 for LM7 and U2OS; ** P <0.01 for MG-63), but not in the already poorly transduced healthy bone cells (NS).

    Article Snippet: Transduction efficiency was analysed after 48 h by fluorescence read-out using an Acumen e X3 microplate cytometer (TTP LabTech Ltd, Melbourn, UK).

    Techniques: Plasmid Preparation, Fluorescence, Transduction, Cytometry, Staining, Expressing, Incubation, Blocking Assay